Part:BBa_K2538005:Design
Tissue inhibitor of metalloproteinases-1 promoter (pTimp1)
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 675
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 587
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 623
Design Notes
Designed using Geneious to meet registry standards. Part was cloned into pSB1C3 using EcoRI and PstI restriction sites.
Source
Murine genomic DNA sequence. Based on Edwards, D. R., Rocheleau, H., Sharma, R. R., Wills, A. J., Cowie, A., Hassell, J. A., & Heath, J. K. (1992). Involvement of AP1 and PEA3 binding sites in the regulation of murine tissue inhibitor of metalloproteinases-1 (TImp1) transcription. Biochimica et Biophysica Acta (BBA)-Gene Structure and Expression, 1171(1), 41-55. https://www.ncbi.nlm.nih.gov/pubmed/1420363/
References
1. Liddelow, Shane A., et al. "Neurotoxic reactive astrocytes are induced by activated microglia." Nature 541.7638 (2017): 481.
2. Chen, Kenian, et al. "RNA-seq characterization of spinal cord injury transcriptome in acute/subacute phases: a resource for understanding the pathology at the systems level." PloS one 8.8 (2013): e72567.
3. Sosunov, Alexander A., et al. "Phenotypic conversions of “protoplasmic” to “reactive” astrocytes in Alexander disease." Journal of Neuroscience 33.17 (2013): 7439-7450.
4. Kim, Jin Hee, et al. "High cleavage efficiency of a 2A peptide derived from porcine teschovirus-1 in human cell lines, zebrafish and mice." PloS one 6.4 (2011): e18556.
5. Farzadfard, Fahim, Samuel D. Perli, and Timothy K. Lu. "Tunable and multifunctional eukaryotic transcription factors based on CRISPR/Cas." ACS synthetic biology 2.10 (2013): 604-613.